Parthenogenetic Activation Induced by Progesterone in Cultured Mouse Oocytes.
نویسندگان
چکیده
منابع مشابه
Parthenogenetic activation of mouse oocytes induced by inhibitors of protein synthesis.
Recently ovulated mouse oocytes at the Metaphase II stage undergo parthenogenetic activation (as indicated by the formation of pronuclei) when incubated for 6 h in the presence of cycloheximide or puromycin; the activation response increases progressively with the concentration of inhibitor. Activation is induced with concentrations of cycloheximide that depress protein synthesis by more than 7...
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Background: Parthenogenesis is the production of an embryo from a female gamete in the absence of any contribution from a male gamete, with or without the eventual development into an adult. In vivo, mammalian parthenogenesis is a rare event. Parthenogenesis can be efficiently induced in vitro with a variety of mechanical, chemical, and electrical stimuli in several species. Hydrostatic pressur...
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This study was designated to clarify the influence of activation of porcine matured oocytes by calcium ionophore on in vitro development of the parthenotes. The follicular oocytes were matured, activated and cultured in North Carolina State University-23 (NCSU-23) medium supplemented with 10% porcine follicular fluid (pFF). The in vitro-matured oocytes were exposed to calcium ionophore at conce...
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The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
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Background: The parthenogenetic of oocytes is important in cloning research, as artificial activation of oocytes is an essential component of nucleus transfer protocols. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial increase observed at fertilization. In...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1995
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.41.7